Product Description
Human LAMa3(Laminin Alpha 3) ELISA Kit is availabe at Gentaur for next week delivery.
Alternative Names: LAM-A3; E170; LAMNA; LOCS; Lama3a; Nicein subunit alpha; Kalinin subunit alpha; Epiligrin subunit alpha
Species: Human
Assay Type: Sandwich
Sensitivity: 6 pg/mL
Standard: 1000 pg/mL
Detection Range: 15.63-1000 pg/mL
Sample Type: Serum, plasma, tissue homogenates and other biological fluids.
Assay Length: 4.5h
Research AreaResearch Area: Signal transduction;Metabolic pathway;Tumor immunity;Developmental science;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Laminin Alpha 3(LAMa3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Laminin Alpha 3(LAMa3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Laminin Alpha 3(LAMa3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Laminin Alpha 3(LAMa3) in the samples is then determined by comparing the OD of the samples to the standard curve.
