Product Description
Human KLK12(Kallikrein 12) ELISA Kit is availabe at Gentaur for next week delivery.
Alternative Names: KLK-L5; KLKL5; Kallikrein-Related Peptidase 12; Kallikrein-like protein 5
Species: Human
Assay Type: Competitive Inhibition
Sensitivity: 7.14 ng/mL
Standard: 1000 ng/mL
Detection Range: 15.63-1000 ng/mL
Sample Type: Serum, plasma, tissue homogenates and other biological fluids.
Assay Length: 2h
Research AreaResearch Area: Enzyme & Kinase;
Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Kallikrein 12(KLK12) protein. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Kallikrein 12(KLK12). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Kallikrein 12(KLK12) in the samples is then determined by comparing the OD of the samples to the standard curve.
