Product Description
Human ITGa11(Integrin Alpha 11) ELISA Kit is availabe at Gentaur for next week delivery.
Alternative Names: ITG-A11
Species: Human
Assay Type: Sandwich
Sensitivity: 0.124 ng/mL
Standard: 20 ng/mL
Detection Range: 0.32-20 ng/mL
Sample Type: Tissue homogenates and other biological fluids.
Assay Length: 4.5h
Research AreaResearch Area: CD & Adhesion molecule;Tumor immunity;Bone metabolism;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Integrin Alpha 11(ITGa11). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Integrin Alpha 11(ITGa11). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Integrin Alpha 11(ITGa11), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Integrin Alpha 11(ITGa11) in the samples is then determined by comparing the OD of the samples to the standard curve.
