Product Description
Human INS(Insulin) ELISA Kit is availabe at Gentaur for next week delivery.
Alternative Names:
Species: Human
Assay Type: Competitive Inhibition
Sensitivity: 25.3 pg/mL
Standard: 5000 pg/mL
Detection Range: 78.13-5000 pg/mL
Sample Type: serum, plasma, cell culture supernates and other biological fluids
Assay Length: 2h
Research AreaResearch Area: Metabolic pathway;Endocrinology;Hormone metabolism;
Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Insulin(INS) protein. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Insulin(INS). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Insulin(INS) in the samples is then determined by comparing the OD of the samples to the standard curve.
