Product Description
Human INPP5D(Inositol Polyphosphate-5-Phosphatase 145kDa) ELISA Kit is availabe at Gentaur for next week delivery.
Alternative Names: SHIP; SHIP1; SIP-145; hp51CN; Phosphatidylinositol-3,4,5-Trisphosphate 5-Phosphatase 1; SH2 domain-containing inositol 5'-phosphatase 1
Species: Human
Assay Type: Sandwich
Sensitivity: 13.5 pg/mL
Standard: 2000 pg/mL
Detection Range: 31.25-2000 pg/mL
Sample Type: Serum, plasma, tissue homogenates and other biological fluids
Assay Length: 4.5h
Research AreaResearch Area: Enzyme & Kinase;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Inositol Polyphosphate-5-Phosphatase 145kDa(INPP5D). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Inositol Polyphosphate-5-Phosphatase 145kDa(INPP5D). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Inositol Polyphosphate-5-Phosphatase 145kDa(INPP5D), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Inositol Polyphosphate-5-Phosphatase 145kDa(INPP5D) in the samples is then determined by comparing the OD of the samples to the standard curve.
