Product Description
Human IL3Ra(Interleukin 3 Receptor Alpha) ELISA Kit is availabe at Gentaur for next week delivery.
Alternative Names: CD123; IL3R-A; IL3RY; IL3RAY; IL3R; IL3RAY; IL3RY; hIL-3Ra
Species: Human
Assay Type: Sandwich
Sensitivity: 0.129 ng/mL
Standard: 20 ng/mL
Detection Range: 0.32-20 ng/mL
Sample Type: serum, plasma, tissue homogenates and other biological fluids
Assay Length: 4.5h
Research AreaResearch Area: Signal transduction;CD & Adhesion molecule;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 3 Receptor Alpha(IL3Ra). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Interleukin 3 Receptor Alpha(IL3Ra). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interleukin 3 Receptor Alpha(IL3Ra), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Interleukin 3 Receptor Alpha(IL3Ra) in the samples is then determined by comparing the OD of the samples to the standard curve.
