Product Description
Human IFIH1(Interferon Induced Helicase C Domain Containing Protein 1) ELISA Kit is availabe at Gentaur for next week delivery.
Alternative Names: Hlcd; IDDM19; MDA5; Helicard; Clinically amyopathic dermatomyositis autoantigen 140 kDa; Melanoma differentiation-associated protein 5; Murabutide down-regulated protein
Species: Human
Assay Type: Sandwich
Sensitivity: 0.115 ng/mL
Standard: 20 ng/mL
Detection Range: 0.32-20 ng/mL
Sample Type: Tissue homogenates, cell lysates and other biological fluids.
Assay Length: 4.5h
Research AreaResearch Area: Signal transduction;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interferon Induced Helicase C Domain Containing Protein 1(IFIH1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Interferon Induced Helicase C Domain Containing Protein 1(IFIH1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interferon Induced Helicase C Domain Containing Protein 1(IFIH1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Interferon Induced Helicase C Domain Containing Protein 1(IFIH1) in the samples is then determined by comparing the OD of the samples to the standard curve.
