Product Description
Human HSP90b1(Heat Shock Protein 90kDa Beta 1) ELISA Kit is availabe at Gentaur for next week delivery.
Alternative Names: HSP90-B1; ECGP; GP96; GRP94; TRA1; Glycoprotein 96; Tumor Rejection Antigen(gp96)1;94 kDa glucose-regulated protein; Endoplasmin
Species: Human
Assay Type: Sandwich
Sensitivity: 0.127 ng/mL
Standard: 20 ng/mL
Detection Range: 0.32-20 ng/mL
Sample Type: serum, plasma, tissue homogenates and other biological fluids
Assay Length: 4.5h
Research AreaResearch Area: Signal transduction;Immune molecule;Developmental science;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Heat Shock Protein 90kDa Beta 1(HSP90b1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Heat Shock Protein 90kDa Beta 1(HSP90b1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Heat Shock Protein 90kDa Beta 1(HSP90b1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Heat Shock Protein 90kDa Beta 1(HSP90b1) in the samples is then determined by comparing the OD of the samples to the standard curve.
