Product Description
Human HSP10(Heat Shock 10kDa Protein 1) ELISA Kit is availabe at Gentaur for next week delivery.
Alternative Names: HSPE1; CPN10; EPF; GROES; Chaperonin 10; Early Pregnancy Factor; 10 kDa heat shock protein, mitochondrial
Species: Human
Assay Type: Sandwich
Sensitivity: 0.59 ng/mL
Standard: 100 ng/mL
Detection Range: 1.57-100 ng/mL
Sample Type: Serum, plasma, tissue homogenates and other biological fluids
Assay Length: 4.5h
Research AreaResearch Area: Signal transduction;Developmental science;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Heat Shock 10kDa Protein 1(HSP10). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Heat Shock 10kDa Protein 1(HSP10). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Heat Shock 10kDa Protein 1(HSP10), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Heat Shock 10kDa Protein 1(HSP10) in the samples is then determined by comparing the OD of the samples to the standard curve.
