Product Description
Human HBg1(Hemoglobin Gamma 1) ELISA Kit is availabe at Gentaur for next week delivery.
Alternative Names: HB-G1; HBGA; HBGR; HSGGL1; Gamma-1-globin
Species: Human
Assay Type: Competitive Inhibition
Sensitivity: 195.5 ng/mL
Standard: 30000 ng/mL
Detection Range: 468.75-30000 ng/mL
Sample Type: Serum, plasma, erythrocyte lysates.
Assay Length: 2h
Research AreaResearch Area: Hematology;
Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Hemoglobin Gamma 1(HBg1) protein. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Hemoglobin Gamma 1(HBg1). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Hemoglobin Gamma 1(HBg1) in the samples is then determined by comparing the OD of the samples to the standard curve.
