Product Description
Human HBF(Fetal Hemoglobin) ELISA Kit is availabe at Gentaur for next week delivery.
Alternative Names: hemoglobin F; Foetal Haemoglobin
Species: Human
Assay Type: Competitive Inhibition
Sensitivity: 0.99 ng/mL
Standard: 200 ng/mL
Detection Range: 3.13-200 ng/mL
Sample Type: serum, plasma, erythrocyte lysates and other biological fluids
Assay Length: 2h
Research AreaResearch Area: Hematology;
Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Fetal Hemoglobin(HBF) protein. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Fetal Hemoglobin(HBF). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Fetal Hemoglobin(HBF) in the samples is then determined by comparing the OD of the samples to the standard curve.
