Product Description
Human HBb(Hemoglobin Beta) ELISA Kit is availabe at Gentaur for next week delivery.
Alternative Names: HB-B; HBD; CD113t-C; Beta Globin; Spinorphin
Species: Human
Assay Type: Competitive Inhibition
Sensitivity: 2.94 μg/mL
Standard: 500 μg/mL
Detection Range: 7.82-500 μg/mL
Sample Type: serum, plasma and erythrocyte lysates
Assay Length: 2h
Research AreaResearch Area: Hematology;
Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Hemoglobin Beta(HBb) protein. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Hemoglobin Beta(HBb). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Hemoglobin Beta(HBb) in the samples is then determined by comparing the OD of the samples to the standard curve.
