Product Description
Human GPR172A(G Protein Coupled Receptor 172A) ELISA Kit is availabe at Gentaur for next week delivery.
Alternative Names: SLC52A2; PAR1; GPCR41; RFT3; Porcine endogenous retrovirus A receptor 1; Riboflavin transporter 3; Solute carrier family 52, riboflavin transporter, member 2
Species: Human
Assay Type: Sandwich
Sensitivity: 0.57 ng/mL
Standard: 100 ng/mL
Detection Range: 1.57-100 ng/mL
Sample Type: Tissue homogenates, saliva and other biological fluids.
Assay Length: 4.5h
Research AreaResearch Area: CD & Adhesion molecule;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to G Protein Coupled Receptor 172A(GPR172A). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to G Protein Coupled Receptor 172A(GPR172A). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain G Protein Coupled Receptor 172A(GPR172A), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of G Protein Coupled Receptor 172A(GPR172A) in the samples is then determined by comparing the OD of the samples to the standard curve.
