Product Description
Human G6PD(Glucose 6 Phosphate Dehydrogenase) ELISA Kit is availabe at Gentaur for next week delivery.
Alternative Names: Glucose-6-phosphate 1-dehydrogenase
Species: Human
Assay Type: Sandwich
Sensitivity: 0.267 ng/mL
Standard: 40 ng/mL
Detection Range: 0.63-40 ng/mL
Sample Type: serum, plasma, tissue homogenates and other biological fluids
Assay Length: 4.5h
Research AreaResearch Area: Metabolic pathway;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glucose 6 Phosphate Dehydrogenase(G6PD). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Glucose 6 Phosphate Dehydrogenase(G6PD). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Glucose 6 Phosphate Dehydrogenase(G6PD), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Glucose 6 Phosphate Dehydrogenase(G6PD) in the samples is then determined by comparing the OD of the samples to the standard curve.
