Product Description
Human FLNb(Filamin B Beta) ELISA Kit is availabe at Gentaur for next week delivery.
Alternative Names: ABP-278; AOI; FH1; FLN1L; LRS1; SCT; TABP; TAP; Actin Binding Protein 278; Actin-binding-like protein; Thyroid autoantigen; Filamin-3; Truncated actin-binding protein
Species: Human
Assay Type: Sandwich
Sensitivity: 5.4 pg/mL
Standard: 1000 pg/mL
Detection Range: 15.63-1000 pg/mL
Sample Type: Tissue homogenates, cell lysates and other biological fluids.
Assay Length: 4.5h
Research AreaResearch Area: Developmental science;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Filamin B Beta(FLNb). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Filamin B Beta(FLNb). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Filamin B Beta(FLNb), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Filamin B Beta(FLNb) in the samples is then determined by comparing the OD of the samples to the standard curve.
