Product Description
Human FLNa(Filamin A Alpha) ELISA Kit is availabe at Gentaur for next week delivery.
Alternative Names: ABP280; FLN-A; ABPX; FLN; FLN1; FMD; MNS; NHBP; OPD; OPD1; OPD2; Actin Binding Protein 280; Endothelial actin-binding protein; Filamin-1; Non-muscle filamin
Species: Human
Assay Type: Sandwich
Sensitivity: 0.65 ng/mL
Standard: 120 ng/mL
Detection Range: 1.88-120 ng/mL
Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length: 4.5h
Research AreaResearch Area: Developmental science;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Filamin A Alpha(FLNa). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Filamin A Alpha(FLNa). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Filamin A Alpha(FLNa), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Filamin A Alpha(FLNa) in the samples is then determined by comparing the OD of the samples to the standard curve.
