Product Description
Human F13B(Coagulation Factor XIII B Polypeptide) ELISA Kit is availabe at Gentaur for next week delivery.
Alternative Names: F13-B; FXIIIB; Fibrin-stabilizing factor B subunit; Protein-glutamine gamma-glutamyltransferase B chain; Transglutaminase B chain
Species: Human
Assay Type: Competitive Inhibition
Sensitivity: 285.1 ng/mL
Standard: 50000 ng/mL
Detection Range: 781.25-50000 ng/mL
Sample Type: plasma
Assay Length: 2h
Research AreaResearch Area: Signal transduction;Metabolic pathway;Hematology;
Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Coagulation Factor XIII B Polypeptide(F13B) protein. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Coagulation Factor XIII B Polypeptide(F13B). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Coagulation Factor XIII B Polypeptide(F13B) in the samples is then determined by comparing the OD of the samples to the standard curve.
