Product Description
Human EPCAM(Epithelial Cell Adhesion Molecule) ELISA Kit is availabe at Gentaur for next week delivery.
Alternative Names: CD326; TACSTD1; EGP; KSA; M4S1; MIC18; TROP1; HEGP2; ESA; Epithelial glycoprotein; Adenocarcinoma-associated antigen; Tumor Associated Calcium Signal Transducer 1
Species: Human
Assay Type: Sandwich
Sensitivity: 12.6 pg/mL
Standard: 2000 pg/mL
Detection Range: 31.25-2000 pg/mL
Sample Type: Serum, plasma, tissue homogenates, cell lysates and other biological fluids.
Assay Length: 4.5h
Research AreaResearch Area: CD & Adhesion molecule;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Epithelial Cell Adhesion Molecule(EPCAM). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Epithelial Cell Adhesion Molecule(EPCAM). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Epithelial Cell Adhesion Molecule(EPCAM), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Epithelial Cell Adhesion Molecule(EPCAM) in the samples is then determined by comparing the OD of the samples to the standard curve.
