Product Description
Human EBLN1(Endogenous Bornavirus Like Nucleoprotein 1) ELISA Kit is availabe at Gentaur for next week delivery.
Alternative Names: Endogenous Borna-Like N Element 1
Species: Human
Assay Type: Sandwich
Sensitivity: 0.061 ng/mL
Standard: 10 ng/mL
Detection Range: 0.16-10 ng/mL
Sample Type: Tissue homogenates, cell lysates and other biological fluids.
Assay Length: 4.5h
Research AreaResearch Area: Infection immunity;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Endogenous Bornavirus Like Nucleoprotein 1(EBLN1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Endogenous Bornavirus Like Nucleoprotein 1(EBLN1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Endogenous Bornavirus Like Nucleoprotein 1(EBLN1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Endogenous Bornavirus Like Nucleoprotein 1(EBLN1) in the samples is then determined by comparing the OD of the samples to the standard curve.
