Product Description
Human DYSF(Dysferlin) ELISA Kit is availabe at Gentaur for next week delivery.
Alternative Names: FER1L1; LGMD2B; Dysferlin,Limb Girdle Muscular Dystrophy 2B(Autosomal Recessive); Fer-1-like protein 1; Dystrophy-associated fer-1-like protein
Species: Human
Assay Type: Sandwich
Sensitivity: 12.5 pg/mL
Standard: 2000 pg/mL
Detection Range: 31.25-2000 pg/mL
Sample Type: Tissue homogenates, cell lysates and other biological fluids
Assay Length: 4.5h
Research AreaResearch Area: Immune molecule;Neuro science;Bone metabolism;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dysferlin(DYSF). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Dysferlin(DYSF). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dysferlin(DYSF), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Dysferlin(DYSF) in the samples is then determined by comparing the OD of the samples to the standard curve.
