Product Description
Human DSG3(Desmoglein 3) ELISA Kit is availabe at Gentaur for next week delivery.
Alternative Names: CDHF6; PVA; 130 kDa pemphigus vulgaris antigen; Cadherin family member 6
Species: Human
Assay Type: Sandwich
Sensitivity: 0.066 ng/mL
Standard: 10 ng/mL
Detection Range: 0.16-10 ng/mL
Sample Type: tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length: 4.5h
Research AreaResearch Area: CD & Adhesion molecule;Infection immunity;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Desmoglein 3(DSG3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Desmoglein 3(DSG3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Desmoglein 3(DSG3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Desmoglein 3(DSG3) in the samples is then determined by comparing the OD of the samples to the standard curve.
