Product Description
Human DEFb103A(Defensin Beta 103A) ELISA Kit is availabe at Gentaur for next week delivery.
Alternative Names: DEFB103; DEFB3; HBD-3; HBD3; HBP-3; HBP3; Beta-defensin 3; Defensin-like protein
Species: Human
Assay Type: Sandwich
Sensitivity: 19.54 pg/mL
Standard: 3000 pg/mL
Detection Range: 46.88-3000 pg/mL
Sample Type: serum, plasma and other biological fluids
Assay Length: 4.5h
Research AreaResearch Area: Infection immunity;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Defensin Beta 103A(DEFb103A). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Defensin Beta 103A(DEFb103A). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Defensin Beta 103A(DEFb103A), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Defensin Beta 103A(DEFb103A) in the samples is then determined by comparing the OD of the samples to the standard curve.
