Product Description
Human CXCL16(Chemokine C-X-C-Motif Ligand 16) ELISA Kit is availabe at Gentaur for next week delivery.
Alternative Names: SR-PSOX; CXCLG16; SRPSOX; Scavenger receptor for phosphatidylserine and oxidized low density lipoprotein; Small-inducible cytokine B16
Species: Human
Assay Type: Sandwich
Sensitivity: 0.262 ng/mL
Standard: 10 ng/mL
Detection Range: 0.16-10 ng/mL
Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length: 4.5h
Research AreaResearch Area: Cytokine;Tumor immunity;Infection immunity;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Chemokine C-X-C-Motif Ligand 16(CXCL16). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Chemokine C-X-C-Motif Ligand 16(CXCL16). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Chemokine C-X-C-Motif Ligand 16(CXCL16), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Chemokine C-X-C-Motif Ligand 16(CXCL16) in the samples is then determined by comparing the OD of the samples to the standard curve.