Product Description
Human CX3CL1(Chemokine C-X3-C-Motif Ligand 1) ELISA Kit is availabe at Gentaur for next week delivery.
Alternative Names: NTN; ABCD3; C3Xkine; CXC3; CXC3C; NTT; SCYD1; ABCD3; FKN; Neurotactin; Fractalkine; Small Inducible Cytokine Subfamily D(Cys-X3-Cys)Member 1
Species: Human
Assay Type: Sandwich
Sensitivity: 0.066 ng/mL
Standard: 10 ng/mL
Detection Range: 0.16-10 ng/mL
Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length: 4.5h
Research AreaResearch Area: Cytokine;Infection immunity;Rheumatology;Autoimmunity;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Chemokine C-X3-C-Motif Ligand 1(CX3CL1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Chemokine C-X3-C-Motif Ligand 1(CX3CL1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Chemokine C-X3-C-Motif Ligand 1(CX3CL1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Chemokine C-X3-C-Motif Ligand 1(CX3CL1) in the samples is then determined by comparing the OD of the samples to the standard curve.
