Product Description
Human CTACK(Cutaneous T-Cell Attracting Chemokine) ELISA Kit is availabe at Gentaur for next week delivery.
Alternative Names: CCL27; ILC; SCYA27; ALP; Skinkine; ESkine; PESKY; CTAK; Chemokine(C-C-Motif)ligand 27; Small Inducible Cytokine Subfamily A Member 27; IL-11 R-alpha-locus chemokine
Species: Human
Assay Type: Sandwich
Sensitivity: 12.9 pg/mL
Standard: 2000 pg/mL
Detection Range: 31.25-2000 pg/mL
Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length: 4.5h
Research AreaResearch Area: Cytokine;Tumor immunity;Infection immunity;Dermatology;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cutaneous T-Cell Attracting Chemokine(CTACK). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cutaneous T-Cell Attracting Chemokine(CTACK). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cutaneous T-Cell Attracting Chemokine(CTACK), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cutaneous T-Cell Attracting Chemokine(CTACK) in the samples is then determined by comparing the OD of the samples to the standard curve.
