Product Description
Human CNDP1(Carnosine Dipeptidase 1) ELISA Kit is availabe at Gentaur for next week delivery.
Alternative Names: CN1; CPGL2; Beta-Ala-His Dipeptidase; Metallopeptidase M20; Carnosinase 1; Serum carnosinase; Glutamate Carboxypeptidase-Like Protein 2
Species: Human
Assay Type: Sandwich
Sensitivity: 0.064 ng/mL
Standard: 10 ng/mL
Detection Range: 0.16-10 ng/mL
Sample Type: Serum, plasma, cerebrospinal fluid and other biological fluids
Assay Length: 4.5h
Research AreaResearch Area: Enzyme & Kinase;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Carnosine Dipeptidase 1(CNDP1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Carnosine Dipeptidase 1(CNDP1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Carnosine Dipeptidase 1(CNDP1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Carnosine Dipeptidase 1(CNDP1) in the samples is then determined by comparing the OD of the samples to the standard curve.
