Product Description
Human CD59(Protectin) ELISA Kit is availabe at Gentaur for next week delivery.
Alternative Names: MIC11; MIN1; MIN2; MIN3; MSK21; MAC-IP; MACIF; MIRL; HRF20; Complement Regulatory Protein; 20 kDa homologous restriction factor; Membrane attack complex inhibition factor
Species: Human
Assay Type: Sandwich
Sensitivity: 5.5 pg/mL
Standard: 800 pg/mL
Detection Range: 12.5-800 pg/mL
Sample Type: Serum, plasma, cell lysates and other biological fluids
Assay Length: 4.5h
Research AreaResearch Area: CD & Adhesion molecule;Tumor immunity;Hematology;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Protectin(CD59). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Protectin(CD59). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Protectin(CD59), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Protectin(CD59) in the samples is then determined by comparing the OD of the samples to the standard curve.
