Product Description
Human CA19-5(Carbohydrate Antigen 19-5) ELISA Kit is availabe at Gentaur for next week delivery.
Alternative Names:
Species: Human
Assay Type: Competitive Inhibition
Sensitivity: 0.86 ng/mL
Standard: 200 ng/mL
Detection Range: 3.13-200 ng/mL
Sample Type: Serum, plasma and other biological fluids.
Assay Length: 2h
Research AreaResearch Area:
Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Carbohydrate Antigen 19-5(CA19-5) protein. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Carbohydrate Antigen 19-5(CA19-5). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Carbohydrate Antigen 19-5(CA19-5) in the samples is then determined by comparing the OD of the samples to the standard curve.
