Product Description
Human bTG(Beta-Thromboglobulin) ELISA Kit is availabe at Gentaur for next week delivery.
Alternative Names: CXCL7; PPBP; PBP; B-TG1; CTAP-III; CTAP3; CTAPIII; LA-PF4; LDGF; MDGF; NAP2; SCYB7; TC1; TC2; TGB1; THBGB1; Pro-Platelet Basic Protein; Chemokine C-X-C-Motif Ligand 7
Species: Human
Assay Type: Sandwich
Sensitivity: 5.9 pg/mL
Standard: 1000 pg/mL
Detection Range: 15.63-1000 pg/mL
Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length: 4.5h
Research AreaResearch Area: Cytokine;Hematology;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Beta-Thromboglobulin(bTG). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Beta-Thromboglobulin(bTG). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Beta-Thromboglobulin(bTG), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Beta-Thromboglobulin(bTG) in the samples is then determined by comparing the OD of the samples to the standard curve.
