Product Description
Human BRAF(B-Raf Proto Oncogene Serine/Threonine Protein Kinase) ELISA Kit is availabe at Gentaur for next week delivery.
Alternative Names: B-raf 1; BRAF1; RAFB1; V-Raf Murine Sarcoma Viral Oncogene Homolog B1
Species: Human
Assay Type: Sandwich
Sensitivity: 0.118 ng/mL
Standard: 20 ng/mL
Detection Range: 0.32-20 ng/mL
Sample Type: tissue homogenates, cell lysates and other biological fluids
Assay Length: 4.5h
Research AreaResearch Area: Enzyme & Kinase;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to B-Raf Proto Oncogene Serine/Threonine Protein Kinase(BRAF). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to B-Raf Proto Oncogene Serine/Threonine Protein Kinase(BRAF). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain B-Raf Proto Oncogene Serine/Threonine Protein Kinase(BRAF), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of B-Raf Proto Oncogene Serine/Threonine Protein Kinase(BRAF) in the samples is then determined by comparing the OD of the samples to the standard curve.
