Product Description
Human BHLHB8(Basic Helix Loop Helix Domain Containing Protein B8) ELISA Kit is availabe at Gentaur for next week delivery.
Alternative Names: MIST1; bHLHa15; BHLHB15; Class A Basic Helix Loop Helix protein 15; Muscle, intestine and stomach expression 1
Species: Human
Assay Type: Sandwich
Sensitivity: 0.071 ng/mL
Standard: 10 ng/mL
Detection Range: 0.16-10 ng/mL
Sample Type: Tissue homogenates and other biological fluids.
Assay Length: 4.5h
Research AreaResearch Area: Signal transduction;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Basic Helix Loop Helix Domain Containing Protein B8(BHLHB8). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Basic Helix Loop Helix Domain Containing Protein B8(BHLHB8). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Basic Helix Loop Helix Domain Containing Protein B8(BHLHB8), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Basic Helix Loop Helix Domain Containing Protein B8(BHLHB8) in the samples is then determined by comparing the OD of the samples to the standard curve.
