Product Description
Human Barbiturates ELISA Kit is availabe at Gentaur for next week delivery.
Alternative Names: Barbituates
Species: Human
Assay Type: Competitive Inhibition
Sensitivity: 0.1 ng/mL
Standard: 25 ng/mL
Detection Range: 0.4-25 ng/mL
Sample Type: Plasma, Serum, Urine
Assay Length: 2h
Research AreaResearch Area: Neuro science;
Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Barbiturates protein. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Barbiturates. Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Barbiturates in the samples is then determined by comparing the OD of the samples to the standard curve.
