Product Description
Human Anti-THSD7A(Anti-Thrombospondin Type I Domain Containing Protein 7A Antibody) ELISA Kit is availabe at Gentaur for next week delivery.
Alternative Names:
Species: Human
Assay Type: Competitive Inhibition
Sensitivity: 1.22 ng/mL
Standard: 200 ng/mL
Detection Range: 3.13-200 ng/mL
Sample Type: serum, plasma and other biological fluids
Assay Length: 2h, 30min
Research AreaResearch Area: Signal transduction;
Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Anti-Thrombospondin Type I Domain Containing Protein 7A Antibody(Anti-THSD7A) protein. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Anti-Thrombospondin Type I Domain Containing Protein 7A Antibody(Anti-THSD7A). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Anti-Thrombospondin Type I Domain Containing Protein 7A Antibody(Anti-THSD7A) in the samples is then determined by comparing the OD of the samples to the standard curve.