Product Description
Human Anti-MBL(Anti-Mannose Binding Lectin Antibody) ELISA Kit is availabe at Gentaur for next week delivery.
Alternative Names: MBL2; COLEC1; HSMBPC; MBP1; MBP; Collectin-1; Mannose-binding protein C; Mannan Binding Protein; Mannose-Binding Lectin(Protein C)2,Soluble(Opsonic Defect)
Species: Human
Assay Type: Competitive Inhibition
Sensitivity: 2.45 ng/mL
Standard: 400 ng/mL
Detection Range: 6.25-400 ng/mL
Sample Type: Serum, plasma and other biological fluids
Assay Length: 2h, 30min
Research AreaResearch Area: Signal transduction;Infection immunity;Immune molecule;
Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Anti-Mannose Binding Lectin Antibody(Anti-MBL) protein. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Anti-Mannose Binding Lectin Antibody(Anti-MBL). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Anti-Mannose Binding Lectin Antibody(Anti-MBL) in the samples is then determined by comparing the OD of the samples to the standard curve.