Product Description
Human Anti-IAA(Anti-Insulin Autoantibody Antibody) ELISA Kit is availabe at Gentaur for next week delivery.
Alternative Names: Anti-Insulin Antibody; Antiinsulin; Insulin Antibodies
Species: Human
Assay Type: Competitive Inhibition
Sensitivity: 1.15 ng/mL
Standard: 200 ng/mL
Detection Range: 3.13-200 ng/mL
Sample Type: Serum, plasma and other biological fluids.
Assay Length: 2h, 30min
Research AreaResearch Area:
Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Anti-Insulin Autoantibody Antibody(Anti-IAA) protein. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Anti-Insulin Autoantibody Antibody(Anti-IAA). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Anti-Insulin Autoantibody Antibody(Anti-IAA) in the samples is then determined by comparing the OD of the samples to the standard curve.
