Product Description
Human AGER(Advanced Glycosylation End Product Specific Receptor) ELISA Kit is availabe at Gentaur for next week delivery.
Alternative Names: RAGE; Receptor For Advanced Glycation Endproducts
Species: Human
Assay Type: Sandwich
Sensitivity: 0.059 ng/mL
Standard: 10 ng/mL
Detection Range: 0.16-10 ng/mL
Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length: 4.5h
Research AreaResearch Area: Metabolic pathway;Tumor immunity;Endocrinology;Cardiovascular biology;Neuro science;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Advanced Glycosylation End Product Specific Receptor(AGER). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Advanced Glycosylation End Product Specific Receptor(AGER). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Advanced Glycosylation End Product Specific Receptor(AGER), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Advanced Glycosylation End Product Specific Receptor(AGER) in the samples is then determined by comparing the OD of the samples to the standard curve.
