Product Description
Human a2PI(Alpha-2-Plasmin Inhibitor) ELISA Kit is availabe at Gentaur for next week delivery.
Alternative Names: SERPINF2; AAP; A2-PI; API; PLI; A2AP; Alpha 2 Antiplasmin; Serpin Peptidase Inhibitor Clade F Member 2; Pigment Epithelium Derived Factor
Species: Human
Assay Type: Sandwich
Sensitivity: 0.69 ng/mL
Standard: 100 ng/mL
Detection Range: 1.57-100 ng/mL
Sample Type: Serum, plasma and other biological fluids.
Assay Length: 4.5h
Research AreaResearch Area: Hematology;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Alpha-2-Plasmin Inhibitor(a2PI). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Alpha-2-Plasmin Inhibitor(a2PI). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Alpha-2-Plasmin Inhibitor(a2PI), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Alpha-2-Plasmin Inhibitor(a2PI) in the samples is then determined by comparing the OD of the samples to the standard curve.
