Product Description
Human 4-Hydroxynonenal (HNE)ELISA Kit is availabe at Gentaur for next week delivery.
Alternative Names: 4-HNE, HNE, 4-hydroxy-2-nonenal, 4-Hydroxynonenal
Species: Human
Assay Type: Competitive Inhibition
Sensitivity: 19.1 pg/mL
Standard: 2500 pg/mL
Detection Range: 39.07-2500 pg/mL
Sample Type: Serum, plasma, tissue homogenates
Assay Length: 2h
Research AreaResearch Area: Signal transduction;Metabolic pathway;Infection immunity;Hormone metabolism;Dermatology;
Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with HNE protein. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to HNE. Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of HNE in the samples is then determined by comparing the OD of the samples to the standard curve.
