Product Description
Histone H4 (Phospho-S47) polyclonal antibody is available at gentaur for next week delivery
Background: Histone proteins H3, H4, H2A, and H2B function as building blocks to package eukaryotic DNA into repeating nucleosome units that are folded in higher order chromatin fibers. The nucleosome is composed of an octamer containing a H3/H4 tetramer and two H2A/H2B dimers, surrounded by approximately 146 base pairs of DNA. A diverse and elaborate array of post-translational modifications including acetylation, phosphorylation, methylation, ubiquitination, and ADP-ribosylation occurs on the N-terminal tail domains of histones. Methylation of position-specific lysine residues in histone N termini is a central modification for regulating epigenetic transitions in chromatin. Each methylatable lysine residue can exist in a mono, di, or tri methylated state. Arginine resdiues can also by mono or di methylated.
Applications: WB IF
Purification&Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage&Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: Histone H4 (Phospho-S47) polyclonal antibody detects endogenous levels of Histone H4 protein only when phosphorylated at Ser47.
W4BiowMW: ~ 11 kDa
Reactivity: Human,Rat
Note: For research use only, not for use in diagnostic procedure.
Immunogen:
Synthetic phosphopeptide derived from human Histone H4 around the phosphorylation site of Serine 47.Alternative Name:
Histone H4; HIST1H4A; H4/A, H4FA; HIST1H4B; H4/I; H4FI; HIST1H4C; H4/G, H4FG; HIST1H4D; H4/B, H4FB; HIST1H4E; H4/J, H4FJ; HIST1H4F; H4/C; H4FC; HIST1H4H; H4/H; H4FH; HIST1H4I; H4/M; H4FM; HIST1H4J; H4/E, H4FE; HIST1H4K; H4/D, H4FD; HIST1H4L; H4/K; H4FK; HIST2H4A; H4/N; H4F2; H4FN; HIST2H4; HIST2H4B; H4/O; H4FO; HIST4H4Western blot analysis: Western blot (WB) analysis of Histone H4 (Phospho-S47) polyclonal antibody at 1:500 dilution LaneA:HEK293T whole cell lysate LaneB:U20S whole cell lysate
Immunohistochemistry:
Immunofluorescence analysis:
Host: Rabbit
Swiss-Prot: P62805
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