Product Description
Histone H3 (AcK64) polyclonal antibody is available at gentaur for next week delivery
Background: In eukaryotes, DNA is wrapped around histone octamers to form the basic unit of chromatin structure. The octamer is composed of histones H2A, H2B, H3 and H4, and it associates with approximately 200 base pairs of DNA to form the nucleosome. The association of DNA with histones results in dense packing of chromatin, which restricts proteins involved in gene transcription from binding to DNA. p300 preferentially acetylates Histone H3 at lysines 14 and 18 and Histone H4 at lysines 5 and 8. PCAF in its native form, primarily acetylates Histone H3 at lysine 14 to a monoacetylated form, and less efficiently acetylates Histone H4 at lysine 8. Histone H4 may also be acetylated at lysines 12 and 16, and the involvement of acetylated H4 with Histones H2A, H2B and H3 suggests that acetylated histones may be involved in dynamic chromatin remodeling.
Applications: WB
Purification&Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage&Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: Histone H3 (AcK64) polyclonal antibody detects endogenous levels of Histone H3 protein.
W4BiowMW: ~ 17 kDa
Reactivity: Human,Mouse,Rat
Note: For research use only, not for use in diagnostic procedure.
Immunogen:
Synthetic peptide, corresponding to Human Histone H3.Alternative Name:
Hist1h3a; Hist1h3b; H3 histone family; member A; H3/A; H31; H3FA; Hist1h3a; HIST1H3B; HIST1H3C; HIST1H3D; HIST1H3E; HIST1H3F; HIST1H3G; HIST1H3H; HIST1H3I; HIST1H3J; histone 1; H3a; Histone cluster 1; H3a; Histone H3.1; Histone H3/a; Histone H3/b; Histone H3/c; Histone H3/d; Histone H3/f; Histone H3/h; Histone H3/i; Histone H3/j; Histone H3/k; Histone H3/lWestern blot analysis: Western blot (WB) analysis of Histone H3 (AcK64) polyclonal antibody at 1:500 dilution LaneA:HEK293T whole cell lysate LaneB:Hela whole cell lysate LaneC:DLD whole cell lysate LaneD:The Heart tissue lysate of Mouse LaneE:The Muscle tissue lysate of Mouse LaneF:The Heart tissue lysate of Rat LaneG:The Muscle tissue lysate of Rat
Immunohistochemistry:
Immunofluorescence analysis:
Host: Rabbit
Swiss-Prot: P68431/Q71DI3/P84243/Q6NXT2
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