Product Description
Histone H3 (Acetyl-K9) polyclonal antibody is available at gentaur for next week delivery
Background: Histone H3 is primarily acetylated at Lys9, 14, 18, 23, 27 and 56. Acetylation of H3 at Lys9 appears to have a dominant role in histone deposition and chromatin assembly in some organisms. Phosphorylation at Ser10, Ser28 and Thr11 of histone H3 is tightly correlated with chromosome condensation during both mitosis and meiosis. Phosphorylation of Thr3 of histone H3 is highly conserved among many species and is catalyzed by the kinase haspin. Immunostaining with phospho-specific antibodies in mammalian cells reveals mitotic phosphorylation of H3 Thr3 in prophase and its dephosphorylation during anaphase.
Applications: WB IHC
Purification&Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage&Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: Ac-Histone H3 (K9) polyclonal antibody detects endogenous levels of Histone H3 protein only when acetylated at K9.
W4BiowMW: ~ 17 kDa
Reactivity: Human,Mouse,Rat
Note: For research use only, not for use in diagnostic procedure.
Immunogen:
Synthetic peptide including acetyl-lysine 9 of Histone H3.Alternative Name:
Histone H3.1; H3/a; H3/b; H3/c; H3/d; H3/f; H3/h; H3/i; H3/j; H3/k; H3/l;Western blot analysis: Western blot (WB) analysis of Histone H3 (Acetyl-K9) pAb at 1:1000 dilution Lane1:3T3-L1 whole cell lysate(30ug) Lane2:C6 whole cell lysate(40ug) Lane3:HEK293T whole cell lysate(30ug) Lane4:A549 whole cell lysate(40ug) Lane5:LOVO whole cell lysate(40ug)
Immunohistochemistry: Immunohistochemistry (IHC) analyzes of Histone H3 (Acetyl-K9) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.
Immunofluorescence analysis:
Host: Rabbit
Swiss-Prot: P68431/Q71DI3/P84243
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