Product Description
His-tag (4C2) monoclonal antibody is available at gentaur for next week delivery
Background: The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. A system that has proven to be very successful relies on the insertion of a six histidine (His6) sequence in the N-terminus of the encoded protein, allowing for efficient coupling to Ni2+-chelating resins and purification by single step affinity chromatography. This polyhistidine sequence can then be removed by specific cleavage at sites recognized by enzymes such as thrombin or enterokinase, permitting the separation of the target protein from the polyhistidine tag.
Applications: WB IP
Purification&Purity: The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage&Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: His-tag (4C2) mAb detects over-expressed or recombinant proteins containing the 6×His epitope tag.
W4BiowMW: N/A
Reactivity: All
Note: For research use only, not for use in diagnostic procedure.
Immunogen:
Synthetic peptide His-tag.Alternative Name:
6 His epitope tag; Hexa His tag; HHHHHH epitope tag; HHHHHH tag; His tagWestern blot analysis: Western blot (WB) analysis of over-expressed His-tagged protein in E. coli, the antibody dilutions are 1:5000 (lane 1) and 1:10000 (lane 2). Each lane was loaded with 10 μg of E. coli lysate.
Immunohistochemistry:
Immunofluorescence analysis: Immunoprecipitation of HEK293T transfected with pcDNA3.1-MCM5-V5-His（48h）whole cell lysate using His-tag mAb (Sepharose Bead Conjugate) #BD0048(lane 2 and lane 3) .Lane 1 is 30% input. The western blot was probed using His-tag #AP0032M. “↑”（supernatant）; “↓(deposition）
Host: Mouse
Swiss-Prot: N/A
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