Product Description
HBB polyclonal antibody is available at gentaur for next week delivery
Background: Hemoglobin (Hgb) is coupled to four iron-binding,methene-linked tetrapyrrole rings(heme). The α (16p13.3; 5'-ζ-pseudoζ-pseudoα2-pseudoα1-α2-α1-θ1-3') and β (11p15.4) globin loci determine the basic hemoglobin structure.The globin portion of Hgb consists of twoαchains and two β chains arranged in pairsforming a tetramer. Each of the four globin chainscovalently associates with a heme group. The bonds between α and β chains are weaker than between similar globin chains, therebyforming a cleavage plane that is important for oxygen binding and release. High affinityfor oxygen occurs upon relaxation of theα1-β2 cleavage plane. When the twoα1-β2 interfaces are closely bound, hemoglobin has a lowaffinityfor oxygen. Hb A,which containstwo α chains plustwo β chains,comprises 97% of totalcirculating hemoglobin. The remaining 3% of totalcirculating hemoglobin iscomprised of Hb A-2,which consists of twoαchains plustwo δ chains, and fetal hemoglobin (Hb F),which consists of two α chainstogetherwith two γ chains.
Applications: WB IHC FC
Purification&Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage&Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: HBB polyclonal antibody detects endogenous levels of HBB protein.
W4BiowMW: ~ 16 kDa
Reactivity: Human,Mouse,Rat
Note: For research use only, not for use in diagnostic procedure.
Immunogen:
Recombinant full length Human HBB.Alternative Name:
Hemoglobin subunit beta; Beta-globin; Hemoglobin beta chain; Cleaved into the following 2 chains:LVV-hemorphin-7; SpinorphinWestern blot analysis: Western blot analysis of extracts of Raji cells, using HBB antibody at 1:500 dilution.
Immunohistochemistry:
Immunofluorescence analysis:
Host: Rabbit
Swiss-Prot: P68871
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