Product Description
HAT1 polyclonal antibody is available at gentaur for next week delivery
Background: In the intact cell, DNA closely associates with histones and other nuclear proteins to form chromatin. The remodeling of chromatin is believed to be a critical component of transcriptional regulation and a major source of this remodeling is brought about by the acetylation of nucleosomal histones. Acetylation of lysine residues in the amino-terminal tail domain of histone results in an allosteric change in the nucleosomal conformation and an increased accessibility to transcription factors by DNA. Conversely, the deacetylation of histones is associated with transcriptional silencing. Several mammalian proteins have been identified as nuclear histone acetylases, including GCN5, PCAF (p300/CBP-associated factor), p300/CBP, HAT1 and the TFIID subunit TAF II p250. Mammalian HDAC1 (also designated HD1), HDAC2 (also designated RPD3) and HDAC3-6 have been identified as histone deacetylases.
Applications: WB
Purification&Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage&Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: HAT1 polyclonal antibody detects endogenous levels of HAT1 protein.
W4BiowMW: ~ 48 kDa
Reactivity: Human,Rat,Mouse
Note: For research use only, not for use in diagnostic procedure.
Immunogen:
Synthetic peptide, corresponding to Human HAT1.Alternative Name:
Histone acetyltransferase 1; HAT1Western blot analysis: Western blot (WB) analysis of HAT1 polyclonal antibody at 1:500 dilution Lane1:EC9706 whole cell lysate(40ug) Lane2:SGC7901 whole cell lysate(40ug) Lane3:HCT116 whole cell lysate(40ug) Lane4:CT-26 whole cell lysate(40ug) Lane5:PC12 whole cell lysate(40ug)
Immunohistochemistry:
Immunofluorescence analysis:
Host: Rabbit
Swiss-Prot: O14929
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