Product Description
Guinea pig IgG2(Immunoglobulin G2) ELISA Kit is availabe at Gentaur for next week delivery.
Alternative Names: IGHG2; Ig Gamma-2 Chain C Region; Immunoglobulin Heavy Constant Gamma 2; G2m Marker; Immunoglobulin Gm2
Species: Guinea pig
Assay Type: Competitive Inhibition
Sensitivity: 124.5 ng/mL
Standard: 25000 ng/mL
Detection Range: 390.63-25000 ng/mL
Sample Type: Serum, plasma and other biological fluids.
Assay Length: 2h
Research AreaResearch Area: Infection immunity;Immune molecule;
Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Immunoglobulin G2(IgG2) protein. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Immunoglobulin G2(IgG2). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Immunoglobulin G2(IgG2) in the samples is then determined by comparing the OD of the samples to the standard curve.
