Product Description
Guinea pig EDN1(Endothelin 1) ELISA Kit is availabe at Gentaur for next week delivery.
Alternative Names: ET1; PPET1; Preproendothelin-1; Big endothelin-1
Species: Guinea pig
Assay Type: Competitive Inhibition
Sensitivity: 2.82 pg/mL
Standard: 500 pg/mL
Detection Range: 7.82-500 pg/mL
Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Assay Length: 2h
Research AreaResearch Area: Endocrinology;Cardiovascular biology;
Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Endothelin 1(EDN1) protein. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Endothelin 1(EDN1). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Endothelin 1(EDN1) in the samples is then determined by comparing the OD of the samples to the standard curve.
