Product Description
Granuphilin polyclonal antibody is available at gentaur for next week delivery
Background: Granuphilin, also designated synaptotagmin-like protein 4 or exophilin 2, is a member of the synaptotagmin-like protein family. In rodents, two isoforms have been characterized as granuphlin-a and granuphilin-b, and alternative splicing in human has also been described. Granuphilin-b is a shorter isoform which contains a different C-terminal sequence than the longer isoform. Granuphilin is part of a ternary complex consisting of Syntaxin 1A (STX1A) and Rab 27A. The interaction between granuphilin and Syntaxin 1A on the plasma membrane is regulated by Rab 27A. Granuphilin is a peripheral membrane protein mainly expressed in pancreatic β-cells and in the pituitary gland. It has been detected on secretory granules and close to the plasma membrane. In the pancreas, granuphilin interacts with Insulin-containing vesicles while in both the pancreas and pituitary, granuphilin modulates the secretion of hormones and the exocytosis of dense-core granules. Overexpression of granuphilin enhances basal Insulin secretion but also inhibits high K+-induced Insulin secretion. The effect of granuphilin on Insulin secretion may be impaired by a mutation that disrupts the binding to either Rab 27A or Syntaxin 1A, making granuphilin a possible regulator in the exocytotic pathway
Applications: WB IHC
Purification&Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage&Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: Granuphilin polyclonal antibody detects endogenous levels of Granuphilin protein.
W4BiowMW: ~ 76 kDa
Reactivity: Human,Mouse,Rat
Note: For research use only, not for use in diagnostic procedure.
Immunogen:
Synthetic peptide, corresponding to Human Granuphilin.Alternative Name:
Synaptotagmin-like protein 4; Exophilin-2; Granuphilin; SYTL4Western blot analysis: Western blot (WB) analysis of Granuphilin polyclonal antibody at 1:500 dilution LaneA:CT26 whole cell lysate
Immunohistochemistry:
Immunofluorescence analysis:
Host: Rabbit
Swiss-Prot: Q96C24
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