Product Description
General VB1(Vitamin B1) ELISA Kit is availabe at Gentaur for next week delivery.
Alternative Names: Thiamine; Thiamin; Aneurine
Species: General
Assay Type: Competitive Inhibition
Sensitivity: 1.63 ng/mL
Standard: 300 ng/mL
Detection Range: 4.69-300 ng/mL
Sample Type: serum, plasma and other biological fluids
Assay Length: 2h
Research AreaResearch Area:
Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Vitamin B1(VB1) protein. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Vitamin B1(VB1). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Vitamin B1(VB1) in the samples is then determined by comparing the OD of the samples to the standard curve.
