Product Description
General SPH(Sphingomyelin) ELISA Kit is availabe at Gentaur for next week delivery.
Alternative Names: SM
Species: General
Assay Type: Competitive Inhibition
Sensitivity: 1.85 μg/mL
Standard: 400 μg/mL
Detection Range: 6.25-400 μg/mL
Sample Type: Serum, plasma and other biological fluids
Assay Length: 2h
Research AreaResearch Area: Signal transduction;Neuro science;
Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Sphingomyelin(SPH) protein. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Sphingomyelin(SPH). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Sphingomyelin(SPH) in the samples is then determined by comparing the OD of the samples to the standard curve.
