Product Description
General rT3(Reverse Triiodothyronine) ELISA Kit is availabe at Gentaur for next week delivery.
Alternative Names: RT3; r-T3; Reverse T3;(2S)-2-Amino-3-[4-(4-Hydroxy-3,5-Diiodophenoxy)-3-Iodophenyl]propanoic Acid; 3,3',5'-Triiodo-L-thyronine
Species: General
Assay Type: Competitive Inhibition
Sensitivity: 27.55 pg/mL
Standard: 6000 pg/mL
Detection Range: 93.75-6000 pg/mL
Sample Type: serum, plasma and other biological fluids
Assay Length: 2h
Research AreaResearch Area: Infection immunity;Endocrinology;Hormone metabolism;Autoimmunity;
Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Reverse Triiodothyronine(rT3) protein. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Reverse Triiodothyronine(rT3). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Reverse Triiodothyronine(rT3) in the samples is then determined by comparing the OD of the samples to the standard curve.
